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Created by Rikas Dzihab from Noun Project
Student Guide

The secret glow of nanobits from the kitchen

What are carbon quantum dots (C-dots)? Why do they behave so differently when exposed to different light sources? How can we observe or change their properties?

  • Provided in kit:
  • 20 g table sugar
  • Small square or rectangular sample holders like spectrophotometer cells
  • Ultraviolet (UV) flashlight or small blacklight
  • Red and green laser pointers
  • Filter papers (or coffee filters)
  • Clear PVA (polyvinyl alcohol) glue
  • Cotton swabs (we used Q-tips)
  • Clean glass microscope slides or small hand-held mirror
  • For extension: Tic-Tac® candies
  • Teacher must provide:
  • Water
  • Measuring equipment (e.g. 10 mL grad. cylinder & scale or eyedroppers & measuring spoons). NOTE: 1 mL is about 20 drops with eye dropper
  • Pyrex glass containers (250 mL beaker or 1 cup measuring cup)
  • Microwave oven
  • Hair dryer
  • For extension: Urea
Intro

Why does lemonade look yellow, but grape juice looks purple? It has to do with the molecules in the juice – the colored materials, or pigments, in lemons and those from grapes are different molecules, and absorb different colors (wavelengths) of light. You see the colors that are not absorbed. The particular wavelengths absorbed or not are a characteristic of the substance and are used as a way to categorize substances. This is another way that scientists determine structure (like what you witnessed in this activity using X-crystallography). Electromagnetic radiation, including visible light, can supply energy, depending on its wavelength, and we can use the energy of light, both absorbed and emitted, to understand something about the molecules in a substance. You are going to do this today!

In this activity, you will use a chemical reaction that converts everyday materials to a substance that glows under special lights. That substance is a type of ‘nanomaterial’ called a ‘carbon quantum dot’ or C-dot, for short. Nanoparticles like C-dots can be billions of times smaller than the diameter of a hair. Nanoparticles are of great interest to scientists because they “fluoresce” (or glow) under ultraviolet light (like a blacklight). The color of the glow can be changed by making the particle larger or smaller, or changing the types of atoms that are in it. Can you make C-dots that glow? What can we do with them? Let’s investigate!

Objectives

By the end of this lesson, you will be able to:

  • Experiment with C-dots to understand how materials can be changed in a chemical reaction
  • View the fluorescence from materials to understand quantized, quantum nature of matter
  • You can create your own objectives too. After reading the introduction, what is your essential question or objective for this activity?


Before the experiment
  • Watch the video of your teacher preparing the C-dot solution. As you watch, write down important procedures they used. Ask any questions you have at this point about the C-dot solution.

  • Read the introduction to get an idea of what you will be doing, and what you are looking for.

  • Listen carefully to your teacher’s instructions, especially about SAFETY!

  • After reading the introduction and objective, write a sentence or two about what you would like to learn or understand after completing this activity. Share with your partner.

Setting up
  • Wear goggles and follow all safety instructions.

  • Your teacher will give you a small amount of the C-dot solution to use at the stations.

  • In your lab notebook, draw the following table. Make sure to leave plenty of space in each box for your writing and observations.

    Depending on what type of dots the teacher makes for the class, or the students make, examples of data tables:


During the experiment

Follow the basic procedure described below. Record the actual amounts that you used. Note colors of materials and other details in each part of the activity – you often don’t recognize what is important until you can compare results!

Darkroom Station
  • Your teacher will provide a sample of non-cooked sugar water solution with no C-dots.

  • Bring the non-C-dot solution up to the UV flashlight. Record your observations in the table.

  • Bring your C-dot solution up to the UV flashlight. Record your observations in the table.

  • Make note of differences between the two.

  • Your teacher will provide a sample of non-cooked sugar water solution with no C-dots.

  • Bring the non-C-dot solution up to the red laser. Record your observations in the table.

  • Bring your C-dot solution up to the red laser. Record your observations in the table.

  • Make note of differences between the two.

  • Your teacher will provide a sample of non-cooked sugar water solution with no C-dots.

  • Bring the non-C-dot solution up to the green laser. Record your observations in the table.

  • Bring your C-dot solution up to the green laser. Record your observations in the table.

  • Make note of differences between the two.

Secret Messages Station
  • Saturate a cotton swab with your C-dot sample.

  • On a piece of filter paper (or notebook paper), use the swab to write your initials (or a short secret message). You may have to re-wet the swab with the C-dots. If the message is wet enough to look dark on the paper, that is probably enough.

  • Allow the marks to dry (use the hair dryer on low if available). When dry, see if you can find the marks in the room’s light.

  • In a darkened room, use the UV flashlight to look for your marks. Can you see them now?

  • Record your observations.

Fingerprint Capture Station
  • In a small disposable container (e.g. plastic weigh boat) add

    a. ~1/4 tsp clear PVA glue (about 20 drops),

    b. 2 drops of your C-dot suspension.

    c. Mix well with the toothpick.

  • Obtain a clean glass microscope slide (only grab by the edges!)

    • Rub your finger or thumb on the skin of your nose or forehead to pick up natural skin oils.
    • Carefully press that finger onto the clean glass surface. (Don’t slide or smear it!)
  • Using a plastic pipette, apply drops of the PVA glue-C-dot mixture to the glass surface, completely covering your fingerprint. Allow to air dry. (You can use the hairdryer with cool air only – don’t heat as the glue may get cloudy.)

  • When the PVA coating is dry, pass your slide to your teacher.

    • They will carefully use the razor blade to scrape up the end of the film, and then carefully peel off the coating.
    • Try not to tear or stretch the film, and only handle its edges.
  • In a darkened room, point the UV flashlight beam at the side of the film that was in contact with the glass.

    • Can you see your glowing fingerprint?
    • How well can you distinguish details?
  • Record your observations.

C-Dot Sample Preparation Station (Optional according to teacher’s needs)
  • Measure out about 1 g of table sugar. Place into a Pyrex container or 250 mL volumetric flask.

  • Add about 10 g of water to the container. Stir until the solid is dissolved. Note the appearance of the sample.

  • Place the container in a microwave oven. Set the microwave to heat at about 40% power for approximately 10 minutes.

  • Start the microwave. Pay careful attention to your experiment!

  • After a few minutes, check the sample. Pause and restart heating as needed.

  • The mixture may begin to smell like burnt sugar.

  • Stop the heating when the mixture has a syrupy orange appearance.

  • The glass will be very hot! Use potholders to remove from the oven.

  • Add about 50 mL of water and swirl until the material is fully dissolved. The solution should be yellowish-orange (lighter than iced tea; if darker, add more water).

  • Describe the product, comparing it to the starting material. Colored or not colored? Clear or cloudy? Thick (“viscous”) or thin?

Analyzing Data

You can try to understand the results of your chemical reactions that formed C-dots by comparing each experiment, either your own or those of your classmates. In the scientific method, results should be replicated with consistency to draw conclusions or make claims about an experiment. Compare your observation table to other students or groups and determine if your results are consistent with theirs.

If you were to change something about this experiment (a variable), what could you change to find out more about C-dots?


Conclusion
  • For each station, write a summary of what you observed or a claim about C-dots based on your observations. Use the following vocabulary words in your summaries:

    • Nanoparticles
    • Fluorescence
    • UV
    • Energy
  • Compare the glow of the C-dots under the UV light, the red laser, and the green laser. How can you explain the differences? What does this show you about how molecules react to different amounts of energy?

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